What is NAD+?
Nicotinamide Adenine Dinucleotide (NAD+) is a vital coenzyme naturally present in every cell of your body. It plays a crucial role in energy production and DNA repair. As we age, the levels of NAD+ in our bodies decline. Supplementing with NAD+ may help improve energy levels, enhance cellular health, and potentially slow down certain aspects of the aging process.
Dosing Schedule
Your NAD+ protocol is designed to gradually increase your dosage over the first few months. This allows your body to adapt to the treatment and helps to minimize potential side effects. Please adhere to the following schedule as prescribed by your healthcare provider.
Month | Weekly Dosage | Insulin Syringe Units |
Month 1 | 20mg/week | 20 units |
Month 2 | 50mg/week | 50 units |
Month 3 | 80mg/week | 80 units |
Month 4+ | 100mg/week | 100 units |
Visual Dosing Guide: How to Measure Your Dose
It is critical to measure your dose accurately. Use the following visual guides to see how much NAD+ to draw into your insulin syringe for each dosage. The conversion is straightforward: 1 unit on the syringe equals 1mg of NAD+.
Month 1: 20mg (20 Units)

Month 2: 50mg (50 Units)

Month 3: 80mg (80 Units)

Month 4 and Beyond: 100mg (100 Units)

Preparing for Your Injection
Proper preparation is key to a safe and successful injection. Follow these steps carefully.
Gather Your Supplies: You will need your vial of NAD+, an insulin syringe, and an alcohol wipe.
Wash Your Hands: Thoroughly wash your hands with soap and water.
Prepare the Vial: If the vial is new, remove the protective cap. Clean the rubber stopper on top of the vial with an alcohol wipe.
Draw the Medication:
Remove the cap from the syringe needle.
Pull the plunger back to the mark that corresponds with your dose (e.g., 20 units for a 20mg dose). This draws air into the syringe.
Insert the needle straight through the center of the rubber stopper on the vial.
Push the plunger to inject the air into the vial. This equalizes the pressure and makes it easier to draw the liquid.
With the needle still in the vial, turn the vial and syringe upside down.
Ensure the tip of the needle is below the surface of the liquid.
Pull the plunger back to draw the correct dose of NAD+ into the syringe.
Check for air bubbles in the syringe. If you see any, gently tap the syringe to make them rise to the top and then push the plunger slightly to expel them. Ensure you still have the correct dose in the syringe.
Once you have the correct dose and no air bubbles, remove the needle from the vial.
How to Administer the Injection
NAD+ is administered as a subcutaneous injection, which means it is injected into the fatty tissue just under the skin.
Select and Clean the Injection Site: Choose an injection site (see guide below). Clean the area with a fresh alcohol wipe and let it air dry.
Pinch the Skin: Gently pinch a 1-2 inch fold of skin at the cleaned injection site.
Insert the Needle: Hold the syringe like a dart and insert the needle at a 90-degree angle into the pinched skin.
Inject the Medication: Slowly and steadily push the plunger all the way down to inject the NAD+.
Withdraw the Needle: Once the syringe is empty, gently pull the needle out at the same angle it was inserted.
Dispose of the Syringe: Immediately place the used syringe and needle into a designated sharps container. Do not recap the needle.
Injection Site Guide

It is important to rotate your injection sites to avoid irritation and ensure proper absorption. The most common sites for subcutaneous injections are the abdomen, thighs, and the back of the upper arms.
Abdomen: Use the area at least 2 inches away from your belly button.
Thigh: Use the front of your thigh.
Upper Arm: Use the fatty tissue on the back of your upper arm.
